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Cat. Number
065916458642460
Chemical Name
Prostaglandin D2 FPIA Kit - Green
References
Synonyms
  • PGD2 Fluorescence Polarization Immunoassay - Green
Stability 1 year
Storage -80°C
Shipping Dry Ice in continental US; may vary elsewhere
Specificity
Prostaglandin D2 100%
Prostaglandin F2α 100%
Thromboxane B2 4.98%
12-HHT 0.71%
Prostaglandin E2 0.25%
6-keto Prostaglandin F1α 0.10%
Arachidonic Acid 0.04%
tetranor-PGEM 0.01%
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Background Reading

Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

Roberts, L.J., and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosis. Prostaglandins 30 383-400 (1985).

Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

Zhang, J., Chung, T.D.Y., and Oldenburg, K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73 (1999).

Ujihara, M., Urade, Y., Eguchi, N., et al. Prostaglandin D2 formation and characterization of its synthetases in various tissues of adult rats. Arch Biochem Biophys 260 521-531 (1988).

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500581-384WELL
384-Well Solid Plate (black; non-binding)
Prostaglandin D2 FPIA Reagent - Green 400 dtn
Prostaglandin D2 FPIA Standard - Green 1 ea
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
500581-1920WELL
384-Well Solid Plate (black; non-binding)
Prostaglandin D2 FPIA Reagent - Green 2000 dtn
Prostaglandin D2 FPIA Standard - Green 1 ea
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
Size Global Purchasing
384 wells  
1920 wells  

Description

Limit of detection: 470 pg/ml · Prostaglandin D2 (PGD2) is biosynthesized in the brain by a glutathione-independent lipocalin-type PGD2 synthase (L-PGDS).1 It accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans.2 PGD2 is also synthesized by mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase (hematopoietic PGD Synthase; H-PGDS).3 In this setting, PGD2 and acts as a pro-inflammatory mediator in allergic reactions following release in large amounts from allergen-stimulated mast cells. Cayman’s PGD2 Fluorescence Polarization Immunoassay (FPIA) is especially designed for High Throughput Screening (HTS) applications for direct, rapid measurement of PGD2. The assay is particularly suited for samples from cell culture and purified enzyme preparations. The PGD2-FPIA is robust (Z' = 0.74), exhibits D120 mP over a range of 244 pg/ml to 1,000 ng/ml PGD2, and has a detection limit of 470 pg/ml.

1 Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

2 Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

3 Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

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