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Cat. Number
065910722081811
Chemical Name
Prostaglandin D2 FPIA Kit - Red
References
Synonyms
  • PGD2 Fluorescence Polarization Immunoassay - Red
Stability 1 year
Storage -80°C
Shipping Dry ice in continental US; may vary elsewhere
Specificity
Prostaglandin F2α 118.46%
Prostaglandin D2 100%
Prostaglandin J2 24.40%
11β-Prostaglandin F2α 4.13%
12(S)-HHTrE 3.58%
Thromboxane B2 3.47%
Prostaglandin E2 2.54%
6-keto Prostaglandin F1α 0.46%
13,14-dihydro-15-keto Prostaglandin D2 0.08%
Arachidonic Acid 0.06%
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Background Reading

Song, W., Wang, M., Ricciotti, E., et al. Tetranor PGDM, an abundant urinary metabolite reflects biosynthesis of prostaglandin D2 in mice and humans. J Biol Chem 283(2) 1179-1188 (2008).

Zhang, J., Chung, T.D.Y., and Oldenburg, K.R. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J Biomol Screen 4(2) 67-73 (1999).

Kanaoka, Y., Ago, H., Inagaki, E., et al. Cloning and crystal structure of hematopoietic prostaglandin D synthase. Cell 90 1085-1095 (1997).

Ujihara, M., Urade, Y., Eguchi, N., et al. Prostaglandin D2 formation and characterization of its synthetases in various tissues of adult rats. Arch Biochem Biophys 260 521-531 (1988).

Fitzpatrick, F.A., and Wynalda, M.A. Albumin-catalyzed metabolism of prostaglandin D2. Identification of products formed in vitro. J Biol Chem 258 11713-11718 (1983).

Satoh, S., Matsumura, H., Nakajima, T., et al. Promotion of sleep by prostaglandin D2 in rats made insomniac by pretreatment with para-chlorophenylalanine. Neurosci Res 21 41-50 (1995).

Urade, Y., Tanaka, T., Eguchi, N., et al. Structural and functional significance of cysteine residues of glutathione-independent prostaglandin D synthase. J Biol Chem 270 1422-1428 (1995).

Roberts, L.J., and Sweetman, B.J. Metabolic fate of endogenously synthesized prostaglandin D2 in a human female with mastocytosis. Prostaglandins 30 383-400 (1985).

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512051-384well
384-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 400 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
512051-96well
96-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 2 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 400 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
512051-1920well
384-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 2000 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
512051-480well
96-Well Solid Plate (black; non-binding)
Foil Plate Covers
FPIA Buffer Concentrate (10X) 4 × 1 ea
Prostaglandin D2 FPIA Reagent - Red 2000 dtn
Prostaglandin D2 FPIA Standard - Red 1 ea
Size Global Purchasing
384 wells  
96 wells  
1920 wells  
480 wells  

Description

Limit of detection: 550 pg/ml · This FPIA is offered with a 96-well plate or a 384-well plate, the other components are all the same between the two sizes. Prostaglandin D2 (PGD2) is biosynthesized in the brain by a soluble, 26 kD glutathione-independent lipocalin-type PGD2 synthase (L-PGDS). PGD2 accumulates in the cerebrospinal fluid (CSF), where it induces physiologic sleep in rats and humans. PGD2 is also synthesized by mast cells and leukocytes by a cellular, myeloid-type, glutathione-dependent PGD synthase (hematopoietic PGD Synthase; H-PGDS). Cayman’s PGD2 Fluorescence Polarization Immunoassay (FPIA) is especially designed for the rapid measurement of PGD2 from cell culture and purified enzyme preparation. The PGD2-FPIA is robust (Z’ = 0.62), exhibits greater than 180 mP over a range of 340 pg/ml to 100 ng/ml PGD2, and has a detection limit of 550 pg/ml.
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