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| Cat. Number | 065882962421834 |
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| Chemical Name | SET7/9 Methyltransferase Inhibitor Screening Assay Kit |
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| References |
Background ReadingLoenen, W.A.M. S- Song, M., and Ghosh, A. FGF2- Couture, J., Collazo, E., Hauk, G., et al. Structural basis for the methylation site specificity of SET7/9. Nat Struct Mol Biol 13(2) 140-146 (2006). Dorgan, K.M., Wooderchak, W.L., Wynn, D.P., et al. An enzyme- Kurash, J.K., Lei, H., Shen, Q., et al. Methylation of p53 by Set7/9 mediates p53 acetylation and activity in vivo. Mol Cell 29 392-400 (2008). Chiang, P.K., Gordon, R.K., Tal, J., et al. S- Xiao, B., Jing, C., Wilson, J.R., et al. Structure and catalytic mechanism of the human histone methyltransferase SET7/9. Nature 421 652-656 (2003). Show all 7 Hide all but first 3
Description
SET7/9 is a MT that acts on various substrates including histone 3 at lysine residue 4 (H3K4), p53, and the transcription factor TAF 10. In Cayman’s SET7/9 MT Inhibitor Screening Assay the transfer of the methyl group from SAM by SET7/9 to the acceptor peptide (TAF 10) generates SAH, which is rapidly converted to urate and H2O2 using an enzyme mixture provided in the kit. A subsequent reaction between H2O2 and ADHP produces the highly fluorescent compound resorufin.
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