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Cat. Number

065840917165146

Chemical Name

sPLA2 (Type V) Inhibitor Screening Assay Kit

References

Synonyms	Secretory Phospholipase A2 (Type V)
Stability	1 year
Storage	-20°C
Shipping	Wet ice in continental US; may vary elsewhere

Background Reading

Dennis, E.A. Phospholipases. Enzymes XVI 307-353 (1983).

Reynolds, L.J., Hughes, L.L., and Dennis, E.A. Analysis of human synovial fluid phospholipase A₂ on short chain phosphatidylcholine-mixed micelles: Development of a spectrophotometric assay suitable for a microtiterplate reader. Anal Biochem 204 190-197 (1992).

Hendrickson, H.S., Hendrickson, E.K., and Dybvig, R.H. Chiral synthesis of a dithiolester analog of phosphatidylcholine as a substrate for the assay of phospholipase A₂. J Lipid Res 24 1532-1537 (1983).

Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A₂ involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A₂ that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

Han, S.K., Kim, K.P., Koduri, R., et al. Roles of Trp31 in high membrane binding and proinflammatory activity of human group V phospholipase A₂. J Biol Chem 274 11881-11888 (1999).

Han, S., Yoon, E.T., and Cho, W. Bacterial expression and characterization of human secretory class V phospholipase A₂. Biochem J 331 353-357 (1998).

Dennis, E.A. Diversity of group types, regulation, and function of phospholipase A₂. J Biol Chem 269 13057-13060 (1994).

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10004883-96WELL
sPLA2 Assay (human Type V)	1 ea
96-Well Solid Plate (Colorimetric Assay)
sPLA2 Assay Buffer (10X)	1 ea
sPLA2 DTNB	4 × 1 ea
sPLA2 Diheptanoyl thio-PC (substrate)	2 × 1 ea

Size	Global Purchasing
96 wells

Description

Phospholipase A₂ (PLA₂) catalyzes the hydrolysis of phospholipids at the sn-2 position yielding a free fatty acid and a lysophospholipid. The release of arachidonic acid from membrane phospholipids by PLA₂ is believed to be a key step in the control of eicosanoid production within the cell.¹ Mammalian tissues contain many secretory PLA₂s (sPLA₂s), including types IB, IIA-F, III, V, X, and XII² of which Type V sPLA₂ is involved in eicosanoid formation in inflammatory cells, such as macrophages and mast cells.^3,4 It has been demonstrated that human type V sPLA₂ can bind phosphatidylcholine (PC) membranes and hydrolyze PC substrates much more efficiently than human type IIA sPLA₂, which makes it better suited for acting on the outer plasma membrane.⁵ Selective targeting and inhibition of sPLA₂s has been problematic as evidenced by the lack of availability of isozyme-specific inhibitors. The Cayman Chemical sPLA₂ (Type V) Inhibitor Screening Assay is a convenient colorimetric assay designed for rapid screening of Type V sPLA₂ inhibitors in a 96 well format. The sPLA₂ (Type V) Inhibitor Screening assay kit includes sufficient human recombinant Type V sPLA₂ for a minimum of 100 reactions. Identification of selective sPLA₂ inhibitors will offer substantial aid in the elucidation of the specific physiological function of each sPLA₂. Each kit contains assay buffer, DTNB, diheptanoyl thio-PC, human sPLA₂, a 96 well plate, plate cover, and complete instructions.

1 Dennis, E.A. Diversity of group types, regulation, and function of phospholipase A₂. J Biol Chem 269 13057-13060 (1994).

2 Balboa, M.A., Balsinde, J., Winstead, M.V., et al. Novel group V phospholipase A₂ involved in arachidonic acid mobilization in murine P388D1 macrophages. J Biol Chem 271 32381-32384 (1996).

3 Reddy, S.T., Winstead, M.V., Tischfield, J.A., et al. Analysis of the secretory phospholipase A₂ that mediates prostaglandin production in mast cells. J Biol Chem 272 13591-13596 (1997).

4 Han, S., Yoon, E.T., and Cho, W. Bacterial expression and characterization of human secretory class V phospholipase A₂. Biochem J 331 353-357 (1998).

5 Han, S.K., Kim, K.P., Koduri, R., et al. Roles of Trp31 in high membrane binding and proinflammatory activity of human group V phospholipase A₂. J Biol Chem 274 11881-11888 (1999).

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