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N-Glycan Sample Preparation

The distribution of N-linked glycans plays a critical role in the efficacy, immunogenicity, and pharmacokinetics of therapeutic proteins. Multi-step, multi-day , sample preparation methods have limited N-linked glycan analysis to only samples that represent critical decision points. Glycan analysis for bioreactor control, strain selection or comparability studies has never been possible.
速度快:
From purification to glycan release and labeling, the entire sample prep can be completed in 3 hours, not 3–4 days.

  • Innovative platform can process up to 192 samples in < 3 hours
  • Modular format enables seamless integration into a variety of workflows
  • Rapid labeling and cleanup for quantitative data

 requires minimal equipment: a centrifuge with a deep-well plate rotor (minimum 44 mm height), a Starter Labware Kit, and Heater and Incubation Blocks.

 

Flexibility

This is modular and can be integrated into any workflow, regardless of sample throughput or sample type. The GlykoPrep platform is a streamlined solution for anyone that requires rapid, quantitative glycan profiling on your current analysis platform.

Applications

  • Cell culture optimization
  • Strain selection
  • Product characterization
  • PAT assays for QbD
  • QC release

Sample Types

  • Monoclonal antibodies (purified or crude)
  • Fc-fusion proteins
  • Other recombinant glycoproteins

Analysis

  • HPLC
  • LC/MS
  • CE
  • MS
  • HPAEC-PAD

Automation Options

  • Manual processing
  • Semi-automated with liquid handling system
  • Fully automated

 

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