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/ Products Classification 点击展开+Prozyme公司于1990年成立于美国加州，专业开发高质量的试剂、试剂盒及平台，为广大客户提供诊断和科研所需的标记物、蛋白质和酶等。Prozyme于2003年1月收购了Glyko®产品线，包括研究糖类的酶及相关试剂。其中包括Glyko®于1999年收购的OxfordGlycoSciences，Ltd.公司的生化试剂产品线。
目前，Prozyme公司可提供商品化的GlykoPrep™样品快速制备平台，该平台大大加速和简化了糖分析样品的制备流程，其制备的高品质产品可用于生化制药药物开发和生产。
1. GlykoPrep™糖样品快速制备平台
治 疗性蛋白质的糖基化谱对其药理学性质发挥着重要作用，会影响免疫原性、药代动力学和药效学。然而，由于目前的手动样品前处理方法复杂、持续多日，只能对开 发流程中代表关键决策点的样品进行分析。为了满足针对生物仿制药和新型生物药物开发日益严格的法规要求，提供更高的数据质量和分析通量速度，ProZyme推出GlykoPrep™ 样品快速制备平台，GlykoPrep-plus先进化学试剂能够实现快速的N-糖链消化、标记和净化，为您提供稳定和可重现的定量结果。
该平台主要以AssayMAP™小柱 为基础，该小柱包含5μl的填充床，可填充粒径在20到100μm之间的几乎所有聚合物、硅胶和凝胶型树脂。样品杯最大样品承载体积为200μl。
AssayMAP™小柱可放置在特殊的模块支架上，结合相关的接收板和收集板（96孔式），完成样品的制备收集。实验时，样品、缓冲液及其它试剂加入到样品杯中，通过微孔板离心机的低速离心处理，溶液流经树脂填充床。两块板同时使用，可最多制备192个样品。
平台特点
· 从纯化到聚糖释放，完成整个样本制备流程just 3h, not 3-4 days
· 非选择性，定量释放及回收完整的N-聚糖
· 选择灵活，可实现高通量（处理1-192个样本）
· 室温标记，无唾液酸损失，标记效率>80%
· 与多种自动化平台的微孔板液体处理系统兼容
|
应用 · 细胞培养优化 · 菌株筛选 · 产品表征 · 质量源于设计中的工艺过程分析 · 质量控制 样本类型 · 单克隆抗体 · Fc融合蛋白 · 其他重组糖蛋白 |
检测方法 · HPLC · LC/MS · CE · MS · HPAEC-PAD 自动化选择 · 手动处理 · 液体处理系统，实现半自动化 · 全自动化 |
平台组成：
· GlykoPrep™Digestion Module
· GlykoPrep™Labeling Module
· GlykoPrep™Cleanup Module
实验流程示意图：
图：GlykoPrep™ Rapid N-Glycan Preparation withInstantAB™实验流程
相关产品链接：
GPPNG-ABGlykoPrep®-plus Rapid N-Glycan Sample Preparation with 2-ABNEW!
GP96NG-AB GlykoPrep™ Rapid N-Glycan Preparation with2-AB
GP96-ABCU GlykoPrep™ Rapid 2-AB Labeling andCleanup
GP96NG-LA GlykoPrep™ Rapid N-Glycan Preparationwith InstantAA™
GP96NG-LB GlykoPrep™ Rapid N-Glycan Preparationwith InstantAB™
GP96NG-LF GlykoPrep™ Rapid N-GlycanPreparation
GP24NG-AB GlykoPrep™ Rapid N-Glycan Preparationwith 2-AB (24-ct)
GP24-ABCU GlykoPrep™Rapid-Reductive-Amination™ 2-AB Labeling and Cleanup(24-ct)
GP24NG-LA GlykoPrep™ Rapid N-Glycan Preparationwith InstantAA™ (24-ct)
GP24NG-LB GlykoPrep™ Rapid N-Glycan Preparationwith InstantAB™
GP24NG-LF GlykoPrep™ Rapid N-Glycan Preparation(24-ct)
Gilson®TOOLKIT
Equipment andSupplies
2.Glyko®糖生物学研究工具
糖苷酶
1.内切糖苷酶
|
Code |
Description |
Size/Qty |
|
N-Glycanase |
||
|
GKE-5006B |
N-Glycanase® (recombinant gene fromElizabethkingia meningoseptica, expressed in E.coli) |
1 ea (200mU in 80µl) |
|
O-Glycanase |
||
|
GK80090 |
Glyko® O-Glycanase (recombinant genefrom Streptococcus pneumoniae, expressed inE.coli) |
1 ea (50 mU (40µl)) |
2. 外切糖苷酶
|
Code |
Description |
Size/Qty |
|
Sialidase |
||
|
GK80043 |
Glyko® Sialidase A™ SamplerKit |
1 ea (1ea) |
|
GK80040 |
Glyko® Sialidase A™ (recombinant gene fromArthrobacter ureafaciens, expressed in E.coli) |
1 ea (1 U (200µl)) |
|
BetaGalactosidase |
||
|
GK80120 |
Glyko® β(1,3,6)-Galactosidase/GALaseII (recomb from Xanthomonas manihotis, expressed in E.coli) |
1 ea (200 mU (40µl)) |
|
Hexosaminidase |
||
|
GKX-5003 |
Glyko® β-N-Acetylhexosaminidase (jackbean)/HEXase III |
1 ea (5 U) |
|
Galactosaminidase |
||
|
GKX-5001 |
Glyko® α-N-Acetylgalactosaminidase(chicken liver) |
1 ea (100mU) |
|
AlphaMannosidase |
||
|
GKX-5010 |
Glyko® α(1-2,3,6)-Mannosidase (jackbean) |
1 ea (10U) |
|
BetaMannosidase |
||
|
GKX-5015 |
Glyko® β-Mannosidase (Helixpomatia) |
1 ea (1 Ulyophilized) |
|
AlphaFucosidase |
||
|
GK80170 |
Glyko® α(1-2)Fucosidase/FUCase II(Xanthomonas manihotis) |
1 ea (4mU) |
· 糖蛋白去糖基化试剂盒
|
Code |
Description |
Size/Qty |
|
EnzymaticDeglycosylation |
||
|
GK80110 |
GlycoProTM Enzymatic DeglycosylationKit for N-Linked & Simple O-Linked Glycans |
1 ea (1 kit (~2 mg ofglycoprotein)) |
|
ChemicalDeglycosylation |
||
|
GKK-500 |
GlycoFreeTM Chemical DeglycosylationKit |
1 ea (1 kit up to 24samples (2 sets of 12)) |
· GlykoScreenTM唾液酸定量检测
|
Code |
Description |
Size/Qty |
|
GS300 |
GlykoScreenTM Rapid Sialic AcidQuantitation Kit |
1 ea (1kit) |
|
GF57 |
Sialic Acid QuantitationKit |
1 ea (kit) |
|
GS300LA |
GlykoScreenTM Sialic AcidQuantitation Kit |
1 ea (1kit) |
|
GS300WD |
GlykoScreenTM Sialic AcidQuantitation Kit |
1 ea (1kit) |
· SignalTM标记试剂盒
|
Code |
Description |
Size/Qty |
|
GKK-402 |
Glyko® Signal™ 2-AA LabelingKit |
1 ea (2 sets (2 x 18rxns)) |
|
GKK-404 |
Glyko® Signal™ 2-AB LabelingKit |
1 ea (2 sets (2 x 18rxns)) |
|
GKK-407 |
Glyko® Signal™ DMB Sialic Acid LabelingKit |
1 ea (2 sets (2 x 10rxns)) |
· 糖类洗涤
|
Code |
Description |
Size/Qty |
|
GlykoClean™ Clean-upStation |
||
|
GC100 |
GlykoClean™ Glycan Clean-upStation |
1 ea (ea) |
|
GlykoClean™ Rapid Clean-UpCartridges |
||
|
GC210 |
GlykoClean™ S-plusCartridges |
1 ea (25-countpack) |
|
GC250 |
GlykoClean™ GCartridges |
1 ea (25-countpack) |
|
GlycoClean™ Carbohydrate Clean-UpCartridges |
||
|
GKI-4025 |
Glyko® GlycoClean™ HCartridges |
1 ea (10cartridges) |
|
GKI-4756 |
Glyko® GlycoClean™ RCartridges |
1 ea (12cartridges) |
|
GKI-4726 |
Glyko® GlycoClean™ SCartridges |
1 ea (12cartridges) |
· 糖标准品
|
Code |
Description |
Size/Qty |
||
|
N-LinkedOligosaccharides |
||||
|
GKC-004301 |
Glyko™ Asialo-, agalacto-,biantennary, core-substituted with fucose(NGA2F) |
1 ea (10 µg) |
||
|
GKC-005300 |
Glyko™ Asialo-, agalacto-,triantennary (NGA3) |
1 ea (10µg) |
||
|
GKC-006300 |
Glyko™ Asialo-, agalacto-,tetraantennary (NGA4) |
1 ea (20µg) |
||
|
GKM-002500 |
Glyko® Oligomannose 5(Man-5) |
1 ea (10µg) |
||
|
GKC-224300 |
Glyko™ Di-sialylated-,galactosylated biantennary (A2) |
1 ea (10 µg (1 x 10µg)) |
||
|
InstantAB™ LabeledOligosaccharides |
||||
|
GKIB-503 |
Glyko® InstantAB™-(GlucoseHomopolymer) |
1 ea (200pmol) |
||
|
GKIB-005 |
Glyko® InstantAB™ Human IgG N-Linked GlycanLibrary |
1 ea(70pmol) |
||
|
GKIB-009 |
Glyko® InstantAB™-(RNAse B N-Linked GlycanLibrary) |
1 ea(70pmol) |
||
|
GKIB-002 |
Glyko® InstantAB™-(Bovine Fetuin N-LinkedGlycan Library) |
1 ea(70pmol) |
||
|
GKIB-001 |
Glyko® InstantAB™-(Human α1-Acid Glycoprotein N-LinkedGlycan Library) |
1 ea (70pmol) |
||
|
InstantAA™ LabeledOligosaccharides |
||||
|
GKIA-503 |
Glyko® InstantAA™ Glucose HomopolymerStandard |
1 ea (200pmol) |
||
|
Hybrid-typeOligosaccharides |
||||
|
GKH-003510 |
Glyko® Hybrid-type N-Glycan withbisecting GlcNAc |
1 ea (10µg) |
||
|
N-Linked Cores |
||||
|
GKR-002000 |
Glyko® Chitobiose(NN) |
1 ea (20µg) |
||
|
GKR-002100 |
Glyko® 4'-β-mannosylchitobiose (MNN,MAN-1) |
1 ea (10µg) |
||
|
GKR-002300 |
Glyko® Conserved trimannosyl core(M3N2, MAN-3) |
1 ea (10µg) |
||
|
Neutral O-LinkedOligosaccharides |
||||
|
GKAD-00125 |
Fucosyl-p-lacto-N-hexaose |
1 ea (100µg) |
||
|
GKAD-00131 |
Glyko® Difucosyllacto-N-hexaose(DFLNH(a)) |
1 ea (100µg) |
||
|
GKAD-00133 |
Glyko® Monofucosyllacto-N-hexaoseIII (MFLNH-III) |
1 ea (100µg) |
||
|
GKAD-00135 |
Glyko® Trifucosyllacto-N-hexaose(TFLNH) |
1 ea (100µg) |
||
|
Sialylated O-LinkedOligosaccharides |
||||
|
GKAD-00116 |
Glyko® 3'-Sialyl-3-fucosyllactose(3'-S,3-FL) |
1 ea (100µg) |
||
|
GKAD-01014 |
Glyko® 3'-Sialyl-N-acetyllactosamine(3'-SLN) |
1 ea (500µg) |
||
|
GKAD-01015 |
Glyko® 6'-Sialyl-N-acetyllactosamine(6'-SLN) |
1 ea (500µg) |
||
|
BloodGroup Oligosaccharides |
||||
|
GKAD-00141 |
Glyko® Blood group Atrisaccharide |
1 ea (5mg) |
||
|
GKAD-00143 |
Glyko® Blood group Btrisaccharide |
1 ea (5mg) |
||
|
GKAD-00144 |
Glyko® Blood group Hdisaccharide |
1 ea (1mg) |
||
|
LewisAntigens |
||||
|
GKAD-00126 |
3'-Sialyl Lewis x |
1 ea (100µg) |
||
|
GKAD-00127 |
Lewis x |
1 ea (100µg) |
||
|
GKAD-00128 |
3'-Sialyl Lewis a |
1 ea (100µg) |
||
|
GKAD-00129 |
Lewis a |
1 ea (100µg) |
||
|
Sialylated Oligosaccharides found inHuman Milk |
||||
|
GKGS-312 |
GlycoSet II |
1 ea (ea ) |
||
|
2-AB(2-Aminobenzamide) Labeled Oligosaccharides |
||||
|
GKSB-103 |
Glyko® 2-AB oligomannose 5 (2-ABMan-5) |
1 ea (100pmol) |
||
|
GKSB-301 |
Glyko® 2-AB asialo-, agalacto-,biantennary (2-AB NGA2) |
1 ea (100pmol) |
||
|
GKSB-304 |
Glyko® 2-AB asialo-, galactosylatedbiantennary (2-AB NA2) |
1 ea (100pmol) |
||
|
GKSB-307 |
Glyko® 2-AB asialo-, agalacto-,triantennary (2-AB NGA3) |
1 ea (100pmol) |
||
|
2-AA(2-Aminobenzoic acid) Labeled Oligosaccharides |
||||
|
GKSA-103 |
Glyko® 2-AA Oligomannose 5 (2-AAMan-5) |
1 ea (100pmol) |
||
|
GKSA-304 |
Glyko® 2-AA asialo, galactosylatedbiantennary (2-AA NA2) |
1 ea (100pmol) |
||
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