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Prozyme公司于1990年成立于美国加州,专业开发高质量的试剂、试剂盒及平台,为广大客户提供诊断和科研所需的标记物、蛋白质和酶等。Prozyme20031月收购了Glyko®产品线,包括研究糖类的酶及相关试剂。其中包括Glyko®1999年收购的OxfordGlycoSciencesLtd.公司的生化试剂产品线。

目前,Prozyme公司可提供商品化的GlykoPrep™样品快速制备平台,该平台大大加速和简化了糖分析样品的制备流程,其制备的高品质产品可用于生化制药药物开发和生产。
       1. GlykoPrep™糖样品快速制备平台
        治 疗性蛋白质的糖基化谱对其药理学性质发挥着重要作用,会影响免疫原性、药代动力学和药效学。然而,由于目前的手动样品前处理方法复杂、持续多日,只能对开 发流程中代表关键决策点的样品进行分析。为了满足针对生物仿制药和新型生物药物开发日益严格的法规要求,提供更高的数据质量和分析通量速度,ProZyme推出GlykoPrep™ 样品快速制备平台,GlykoPrep-plus先进化学试剂能够实现快速的N-糖链消化、标记和净化,为您提供稳定和可重现的定量结果。
     
        该平台主要以AssayMAP™小柱 为基础,该小柱包含5μl的填充床,可填充粒径在20100μm之间的几乎所有聚合物、硅胶和凝胶型树脂。样品杯最大样品承载体积为200μl。 
        AssayMAP™小柱可放置在特殊的模块支架上,结合相关的接收板和收集板(96孔式),完成样品的制备收集。实验时,样品、缓冲液及其它试剂加入到样品杯中,通过微孔板离心机的低速离心处理,溶液流经树脂填充床。两块板同时使用,可最多制备192个样品。

 

平台特点

·        从纯化到聚糖释放,完成整个样本制备流程just 3h, not 3-4 days

·        非选择性,定量释放及回收完整的N-聚糖

·        选择灵活,可实现高通量(处理1-192个样本)

·        室温标记,无唾液酸损失,标记效率>80%

·        与多种自动化平台的微孔板液体处理系统兼容


 

应用

·        细胞培养优化

·        菌株筛选

·        产品表征

·        质量源于设计中的工艺过程分析

·        质量控制

样本类型

·        单克隆抗体

·        Fc融合蛋白

·        其他重组糖蛋白

检测方法

·        HPLC

·        LC/MS

·        CE

·        MS

·        HPAEC-PAD

自动化选择

·        手动处理

·        液体处理系统,实现半自动化

·        全自动化

平台组成:

·         GlykoPrep™Digestion Module

·         GlykoPrep™Labeling Module

·         GlykoPrep™Cleanup Module

实验流程示意图:


图:GlykoPrepRapid N-Glycan Preparation withInstantAB™实验流程

相关产品链接:

GPPNG-ABGlykoPrep®-plus Rapid N-Glycan Sample Preparation with 2-ABNEW!

GP96NG-AB GlykoPrep™ Rapid N-Glycan Preparation with2-AB

GP96-ABCU GlykoPrep™ Rapid 2-AB Labeling andCleanup

GP96NG-LA GlykoPrep™ Rapid N-Glycan Preparationwith InstantAA

GP96NG-LB GlykoPrep™ Rapid N-Glycan Preparationwith InstantAB

GP96NG-LF GlykoPrep™ Rapid N-GlycanPreparation

GP24NG-AB GlykoPrep™ Rapid N-Glycan Preparationwith 2-AB (24-ct)

GP24-ABCU GlykoPrep™Rapid-Reductive-Amination™ 2-AB Labeling and Cleanup(24-ct)

GP24NG-LA GlykoPrep™ Rapid N-Glycan Preparationwith InstantAA™ (24-ct)

GP24NG-LB GlykoPrep™ Rapid N-Glycan Preparationwith InstantAB

GP24NG-LF GlykoPrep™ Rapid N-Glycan Preparation(24-ct)

Gilson®TOOLKIT

Equipment andSupplies

2.Glyko®糖生物学研究工具

糖苷酶

1.内切糖苷酶

Code

Description

Size/Qty

N-Glycanase

GKE-5006B

N-Glycanase® (recombinant gene fromElizabethkingia meningoseptica, expressed in E.coli)

1 ea (200mU in 80µl)

O-Glycanase

GK80090

Glyko® O-Glycanase (recombinant genefrom Streptococcus pneumoniae, expressed inE.coli)

1 ea (50 mU (40µl))


2. 外切糖苷酶

Code

Description

Size/Qty

Sialidase

GK80043

Glyko® Sialidase A™ SamplerKit

1 ea (1ea)

GK80040

Glyko® Sialidase A(recombinant gene fromArthrobacter ureafaciens, expressed in E.coli)

1 ea (1 U (200µl))

BetaGalactosidase

GK80120

Glyko® β(1,3,6)-Galactosidase/GALaseII (recomb from Xanthomonas manihotis, expressed in E.coli)

1 ea (200 mU (40µl))

Hexosaminidase

GKX-5003

Glyko® β-N-Acetylhexosaminidase (jackbean)/HEXase III

1 ea (5 U)

Galactosaminidase

GKX-5001

Glyko® α-N-Acetylgalactosaminidase(chicken liver)

1 ea (100mU)

AlphaMannosidase

GKX-5010

Glyko® α(1-2,3,6)-Mannosidase (jackbean)

1 ea (10U)

BetaMannosidase

GKX-5015

Glyko® β-Mannosidase (Helixpomatia)

1 ea (1 Ulyophilized)

AlphaFucosidase

GK80170

Glyko® α(1-2)Fucosidase/FUCase II(Xanthomonas manihotis)

1 ea (4mU)

 

·         糖蛋白去糖基化试剂盒

Code

Description

Size/Qty

EnzymaticDeglycosylation

GK80110

GlycoProTM Enzymatic DeglycosylationKit for N-Linked & Simple O-Linked Glycans

1 ea (1 kit (~2 mg ofglycoprotein))

ChemicalDeglycosylation

GKK-500

GlycoFreeTM Chemical DeglycosylationKit

1 ea (1 kit up to 24samples (2 sets of 12))

 

·         GlykoScreenTM唾液酸定量检测

Code

Description

Size/Qty

GS300

GlykoScreenTM Rapid Sialic AcidQuantitation Kit

1 ea (1kit)

GF57

Sialic Acid QuantitationKit

1 ea (kit)

GS300LA

GlykoScreenTM Sialic AcidQuantitation Kit

1 ea (1kit)

GS300WD

GlykoScreenTM Sialic AcidQuantitation Kit

1 ea (1kit)

 

·         SignalTM标记试剂盒

Code

Description

Size/Qty

GKK-402

Glyko® Signal™ 2-AA LabelingKit

1 ea (2 sets (2 x 18rxns))

GKK-404

Glyko® Signal™ 2-AB LabelingKit

1 ea (2 sets (2 x 18rxns))

GKK-407

Glyko® Signal™ DMB Sialic Acid LabelingKit

1 ea (2 sets (2 x 10rxns))

 

·         糖类洗涤

Code

Description

Size/Qty

GlykoCleanClean-upStation

GC100

GlykoClean™ Glycan Clean-upStation

1 ea (ea)

GlykoCleanRapid Clean-UpCartridges

GC210

GlykoClean™ S-plusCartridges

1 ea (25-countpack)
4 ea (25-countpack)

GC250

GlykoClean™ GCartridges

1 ea (25-countpack)
4 ea (25-countpack)

GlycoCleanCarbohydrate Clean-UpCartridges

GKI-4025

Glyko® GlycoClean™ HCartridges

1 ea (10cartridges)

GKI-4756

Glyko® GlycoCleanRCartridges

1 ea (12cartridges)

GKI-4726

Glyko® GlycoClean™ SCartridges

1 ea (12cartridges)

 

·         糖标准品

Code

Description

Size/Qty

N-LinkedOligosaccharides

GKC-004301

Glyko™ Asialo-, agalacto-,biantennary, core-substituted with fucose(NGA2F)

1 ea (10 µg)
2 ea (20 µg (2x 10 µg) )

GKC-005300

Glyko™ Asialo-, agalacto-,triantennary (NGA3)

1 ea (10µg)
2 ea (20µg)

GKC-006300

Glyko™ Asialo-, agalacto-,tetraantennary (NGA4)

1 ea (20µg)

GKM-002500

Glyko® Oligomannose 5(Man-5)

1 ea (10µg)
2 ea (20 µg (2x 10 µg)µ)

GKC-224300

Glyko™ Di-sialylated-,galactosylated biantennary (A2)

1 ea (10 µg (1 x 10µg))
2 ea (10 µg)

InstantABLabeledOligosaccharides

GKIB-503

Glyko® InstantAB™-(GlucoseHomopolymer)

1 ea (200pmol)

GKIB-005

Glyko® InstantAB™ Human IgG N-Linked GlycanLibrary

1 ea(70pmol)

GKIB-009

Glyko® InstantAB™-(RNAse B N-Linked GlycanLibrary)

1 ea(70pmol)

GKIB-002

Glyko® InstantAB™-(Bovine Fetuin N-LinkedGlycan Library)

1 ea(70pmol)

GKIB-001

Glyko® InstantAB™-(Human α1-Acid Glycoprotein N-LinkedGlycan Library)

1 ea (70pmol)

InstantAALabeledOligosaccharides

GKIA-503

Glyko® InstantAA™ Glucose HomopolymerStandard

1 ea (200pmol)

Hybrid-typeOligosaccharides

GKH-003510

Glyko® Hybrid-type N-Glycan withbisecting GlcNAc

1 ea (10µg)

N-Linked Cores

GKR-002000

Glyko® Chitobiose(NN)

1 ea (20µg)

GKR-002100

Glyko® 4'-β-mannosylchitobiose (MNN,MAN-1)

1 ea (10µg)

GKR-002300

Glyko® Conserved trimannosyl core(M3N2, MAN-3)

1 ea (10µg)
2 ea (20 µg (2x 10 µg))

Neutral O-LinkedOligosaccharides

GKAD-00125

Fucosyl-p-lacto-N-hexaose

1 ea (100µg)

GKAD-00131

Glyko® Difucosyllacto-N-hexaose(DFLNH(a))

1 ea (100µg)

GKAD-00133

Glyko® Monofucosyllacto-N-hexaoseIII (MFLNH-III)

1 ea (100µg)

GKAD-00135

Glyko® Trifucosyllacto-N-hexaose(TFLNH)

1 ea (100µg)

Sialylated O-LinkedOligosaccharides

GKAD-00116

Glyko® 3'-Sialyl-3-fucosyllactose(3'-S,3-FL)

1 ea (100µg)

GKAD-01014

Glyko® 3'-Sialyl-N-acetyllactosamine(3'-SLN)

1 ea (500µg)

GKAD-01015

Glyko® 6'-Sialyl-N-acetyllactosamine(6'-SLN)

1 ea (500µg)

BloodGroup Oligosaccharides

   

GKAD-00141

Glyko® Blood group Atrisaccharide

1 ea (5mg)

   

GKAD-00143

Glyko® Blood group Btrisaccharide

1 ea (5mg)

   

GKAD-00144

Glyko® Blood group Hdisaccharide

1 ea (1mg)

   

LewisAntigens

   

GKAD-00126

3'-Sialyl Lewis x

1 ea (100µg)

   

GKAD-00127

Lewis x

1 ea (100µg)

   

GKAD-00128

3'-Sialyl Lewis a

1 ea (100µg)

   

GKAD-00129

Lewis a

1 ea (100µg)

   

Sialylated Oligosaccharides found inHuman Milk

   

GKGS-312

GlycoSet II

1 ea (ea )

   

2-AB(2-Aminobenzamide) Labeled Oligosaccharides

   

GKSB-103

Glyko® 2-AB oligomannose 5 (2-ABMan-5)

1 ea (100pmol)
6 ea (100pmol)

 

GKSB-301

Glyko® 2-AB asialo-, agalacto-,biantennary (2-AB NGA2)

1 ea (100pmol)
6 ea (100pmol)

   

GKSB-304

Glyko® 2-AB asialo-, galactosylatedbiantennary (2-AB NA2)

1 ea (100pmol)
6 ea (100pmol)

   

GKSB-307

Glyko® 2-AB asialo-, agalacto-,triantennary (2-AB NGA3)

1 ea (100pmol)
6 ea (100pmol)

   

2-AA(2-Aminobenzoic acid) Labeled Oligosaccharides

   

GKSA-103

Glyko® 2-AA Oligomannose 5 (2-AAMan-5)

1 ea (100pmol)
6 ea (100pmol)

   

GKSA-304

Glyko® 2-AA asialo, galactosylatedbiantennary (2-AA NA2)

1 ea (100pmol)
6 ea (100pmol)